A genetic system yields self-cleaving inteins for bioseparations DW Wood, W Wu, G Belfort, V Derbyshire, M Belfort Nature biotechnology 17 (9), 889-892, 1999 | 342 | 1999 |
Simple bioseparations using self-cleaving elastin-like polypeptide tags MR Banki, L Feng, DW Wood Nature methods 2 (9), 659-662, 2005 | 314 | 2005 |
Genetic definition of a protein-splicing domain: functional mini-inteins support structure predictions and a model for intein evolution V Derbyshire, DW Wood, W Wu, JT Dansereau, JZ Dalgaard, M Belfort Proceedings of the National Academy of Sciences 94 (21), 11466-11471, 1997 | 201 | 1997 |
Novel and economical purification of recombinant proteins: intein‐mediated protein purification using in vivo polyhydroxybutyrate (PHB) matrix association MR Banki, TU Gerngross, DW Wood Protein science 14 (6), 1387-1395, 2005 | 192 | 2005 |
UV-Assisted Graft Polymerization of N-vinyl-2-pyrrolidinone onto Poly(ether sulfone) Ultrafiltration Membranes: Comparison of Dip versus Immersion Modification … J Pieracci, DW Wood, JV Crivello, G Belfort Chemistry of Materials 12 (8), 2123-2133, 2000 | 174 | 2000 |
Intein applications: from protein purification and labeling to metabolic control methods DW Wood, JA Camarero Journal of Biological Chemistry 289 (21), 14512-14519, 2014 | 167 | 2014 |
New trends and affinity tag designs for recombinant protein purification DW Wood Current opinion in structural biology 26, 54-61, 2014 | 155 | 2014 |
Optimized single‐step affinity purification with a self‐cleaving intein applied to human acidic fibroblast growth factor DW Wood, V Derbyshire, W Wu, M Chartrain, M Belfort, G Belfort Biotechnology progress 16 (6), 1055-1063, 2000 | 149 | 2000 |
Regulation of protein activity with small‐molecule‐controlled inteins G Skretas, DW Wood Protein Science 14 (2), 523-532, 2005 | 134 | 2005 |
The potential role of self-cleaving purification tags in commercial-scale processes BA Fong, WY Wu, DW Wood Trends in biotechnology 28 (5), 272-279, 2010 | 131 | 2010 |
American Burn Association/Shriners Hospitals for Children burn outcomes questionnaire: construction and psychometric properties LH Daltroy, MH Liang, CB Phillips, MB Daugherty, M Hinson, M Jenkins, ... The Journal of burn care & rehabilitation 21 (1), 29-39, 2000 | 114 | 2000 |
Optimization of ELP-intein mediated protein purification by salt substitution BA Fong, WY Wu, DW Wood Protein expression and purification 66 (2), 198-202, 2009 | 106 | 2009 |
Recombinant protein purification by self-cleaving aggregation tag WY Wu, C Mee, F Califano, R Banki, DW Wood Nature protocols 1 (5), 2257-2262, 2006 | 102 | 2006 |
Point-of-care production of therapeutic proteins of good-manufacturing-practice quality R Adiga, M Al-Adhami, A Andar, S Borhani, S Brown, D Burgenson, ... Nature Biomedical Engineering 2 (9), 675-686, 2018 | 97 | 2018 |
Inteins and affinity resin substitutes for protein purification and scale up MR Banki, DW Wood Microbial Cell Factories 4, 1-6, 2005 | 96 | 2005 |
Cell-free protein synthesis approach to biosensing hTRβ-specific endocrine disruptors ASM Salehi, MJ Shakalli Tang, MT Smith, JM Hunt, RA Law, DW Wood, ... Analytical chemistry 89 (6), 3395-3401, 2017 | 93 | 2017 |
Integrated Recombinant Protein Expression and Purification Platform Based on Ralstonia eutropha GC Barnard, JD McCool, DW Wood, TU Gerngross Applied and environmental microbiology 71 (10), 5735-5742, 2005 | 84 | 2005 |
Expression and purification of ELP-intein-tagged target proteins in high cell density E. coli fermentation BA Fong, DW Wood Microbial cell factories 9, 1-11, 2010 | 83 | 2010 |
Intein‐mediated purification of cytotoxic endonuclease I‐ Tev I by insertional inactivation and pH‐controllable splicing W Wu, DW Wood, G Belfort, V Derbyshire, M Belfort Nucleic acids research 30 (22), 4864-4871, 2002 | 77 | 2002 |
A dual ELP-tagged split intein system for non-chromatographic recombinant protein purification C Shi, Q Meng, DW Wood Applied microbiology and biotechnology 97, 829-835, 2013 | 75 | 2013 |